In addition, it highlights key model requirements and remaining challenges for human iPSC-derived BBB models in vitro.īlood-brain barrier Brain endothelial cells iPSC in vitro model.Ĭopyright © 2020 The Authors.
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This review summarizes important functions of the BBB and current efforts to create iPSC-derived BBB models in both static and dynamic conditions. Currently, many different models of the BBB using iPSC-derived cells are under evaluation to study BBB formation, maintenance, disruption, drug transport and diseases affecting the BBB. iPSC-derived brain endothelial cells form tight cell layers in vitro and their barrier properties can be enhanced through coculture with other cell types of the BBB. Given the possibility to generate large amounts of human iPSC-derived brain endothelial cells they are a feasible alternative when modelling the BBB in vitro. Human-induced pluripotent stem cell (iPSC)-derived brain endothelial cells have proven a promising alternative source of brain endothelial-like cells that replicate tight cell layers with low paracellular permeability. Unfortunately, many cell lines and primary cells do not recreate physiological restriction of transport in vitro. Currently used in vitro models of the BBB include immortalized brain endothelial cell lines and primary brain endothelial cells of human and animal origin. Furthermore, BBB models are important tools in drug development and support the evaluation of the brain-penetrating properties of novel drug molecules. Models of the BBB are useful for studying the development and maintenance of the BBB as well as diseases affecting it.
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Its primary function is to maintain the tightly controlled microenvironment of the brain. The blood-brain barrier (BBB) constitutes the interface between the blood and the brain tissue.
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3 Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden. Heart of Giving Podcast, Kimberly Bryant.Electronic address: 2 Division of Micro and Nanosystems, KTH Royal Institute of Technology, Stockholm, Sweden AIMES, Department of Neuroscience, Karolinska Institute, Stockholm, Sweden. 1 Institute of Neuroscience and Physiology, Department of Neurochemistry, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden Systems Biology Research Centre, School of Bioscience, University of Skövde, Skövde, Sweden Discovery Biology, Discovery Sciences, R&D, AstraZeneca, Mölndal, Sweden.